The physico-chemical characters of the New Calabar-Bonny River were investigated for two years to determine its water quality for aquaculture production and human domestic uses. Three stations were chosen to check for the variations in the selected waters parameters.  pH, Temperature, salinity, Dissolved oxygen, Alkalinity, Conductivity and Turbidity were analysed monthly from June 2011 to May 2013 using standard method s and procedures. The ranges obtained were found to be comparable to other Nigerian and African Reservoirs. Mean values obtained were: temperature 28.62±0.62^oC, 28.37±0.7^oC, 28.41±0.57^oC, pH 6.36±0.34, 6.19±38, 2.27±0.74, salinity 11.50±7.71 ^o/oo, 12.15±5.24 ^o/oo, 0.58±0.94 ^o/oo, and dissolved oxygen 6.88±0.51mg/l, 6.92±0.58mg/l, 6.88±0.63mg/l for stations 1, station 2 and station 3 respectively. There was significant difference in salinity level between the three stations, revealing that station 1 is a salt water area, station 2 is brackish/tidal and station 3 is predominantly fresh water. Alkalinity had a mean of 12.61 ± 1.19mg/l. Station 2 recorded the highest alkalinity level (13.60 ± 8.86mg/l) while station 3 recorded the lowest (10.93 ± 10.37 mg/l). Station 1 had the highest mean TDS concentration (10128.2 ± 9069.11 ppm) while station 3 had the lowest mean TDS concentration (2732.90 ± 2644.01 ppm). Mean conductivity was 3752.74 ± 257.58µs/cm. An annual mean of Turbidity 9.81 ± 3.74 NTU was recorded.  Station 3 recorded the lowest mean turbidity of 5.12 ± 3.55 NTU while station 1 recorded the highest mean turbidity of 14.29 ± 8.76 NTU. Turbidity was low in the dry season and high in the rainy season. This study concludes that the physico-chemical parameters of water of the New Calabar-Bonny River meet the recommended and obtainable state of the aquaculture production and for human consumption.

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