Mr Otarigho Benson and Falade, M.O., Opene, A.J.   (Published 2016)

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Falade, M.O., Opene, A.J. and Benson, O., 2016. DNA barcoding of Clarias gariepinus, Coptodon zillii and Sarotherodon melanotheron from Southwestern Nigeria. F1000Research, 5.



DNA barcoding has been adopted as a gold standard rapid, precise and unifying identification system for animal species and provides a database of genetic sequences that can be used as a tool for universal species identification. In this study, we employed mitochondrial genes 16S rRNA (16S) and cytochrome oxidase subunit I (COI) for the identification of some Nigerian freshwater catfish and Tilapia species. Approximately 655 bp were amplified from the 5? region of the mitochondrial cytochrome C oxidase subunit I (COI) gene whereas 570 bp were amplified for the 16S rRNA gene. Nucleotide divergences among sequences were estimated based on Kimura 2-parameter distances and the genetic relationships were assessed by constructing phylogenetic trees using the neighbour-joining (NJ) and maximum likelihood (ML) methods. Analyses of consensus barcode sequences for each species, and alignment of individual sequences from within a given species revealed highly consistent barcodes (99% similarity on average), which could be compared with deposited sequences in public databases. The nucleotide distance between species belonging to different genera based on COI ranged from 0.17% between Sarotherodon melanotheron and Coptodon zillii to 0.49% between Clarias gariepinus and C. zillii, indicating that S. melanotheron and C. zillii are closely related. Based on the data obtained, the utility of COI gene was confirmed in accurate identification of three fish species from Southwest Nigeria

Item Type: Journal article(non-copyrighted)
Format: PDF document,   2.67 MB
Copyright: Creative Commons LicenseCreative Commons license
Keywords: DNA barcoding
Department: Natural Science
Field of Study: Animal Science
Uploaded By: Uddin Osemengbe Oyaimare
Date Added: 02 Nov 2017 9:33am
Last Modified: 23 Nov 2017
Journal URL: https://www.edouniversity.edu.ng/oer/journal/dna_barcoding_of_clarias_gariepinus_coptodon_zillii_and_sarotherodon_melanotheron_from_southwestern_nigeria

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