This study was carried out to determined the efficacy of freshly prepared pestal granules containing the wild and mutant strains of Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae in controlling weeds in a potted experiment. Six multi-combination formulations of the wild and mutant strains of Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae were combined with a semolina kaolin granular formulation which were represented as follows: BHI, BH2, BH3, BH4, BH5, and BH6. The effect of each formulated pesta granules were supplemented with an adjuvants (glycerol, glucose, sucrose, fructose, dextrose, lactose sugar, peptone) and they were evaluated on Tridax procumbens and sorghum plant. The efficacy of the bioherbicides were in the following orders: BH4>BH2>BH6>BH3>BH1>BH5. BH4 showed the highest activity compared to other formulations by reducing Tridax procumbens to the lowest dry weights and showing the highest percentage disease severity on Tridax procumbens compared to other formulations. BH4 among other formulations also lead to increase in the amount of grain yield and 100-grain weight of sorghum plants compared to uninoculated plants. Therefore, the use of pesta granules containing wild and mutant strains of Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae exposed to U.V for 1 hour 30 minutes against weeds using Tridax procumbens as a study case could help in achieving a sustainable agriculture through the application of bioherbicide produced during this study .