The anxiolytic effect of the leaves of water lily was investigated using mice as experimental
animals. Thirty (30) were mice randomly divided into group 1, 2 and 3. Before the neurobehavioral
parameters were assessed, the LD50 and the phytochemical screening of the leaves of the plant
were determined. The open field maze and the transition box apparatus were used to determine
the level of fear and anxiety related behaviors in mice. In the open field arena, the grooming
frequency was statistically not different when compared to control. Stretch attend posture
frequency (SAP) was statistically higher in the test groups when compared to control (P<0.001).
It was also observed that the frequency of defecation decreased in the treated groups when
compared to the control group (P<0.01). The light duration box and frequency of transition were
statistically higher (P<0.001) compared to control. Thus, the leaves of the plant tend to reduce
the level anxiety in mice.