Edem VF, Akinyoola SB, Olaniyi JA, Rahamon SK, Owoeye O, Arinola OG. Haematological Parameters of Wister Rats Exposed to 2, 2 Dichlorovinyl Dimethyl Phosphate Chemical. Asian J Exp Biol Sci 2012; 3(4): 838-841.

ABSTRACT

Haematological indices were determined in 21 male Wistar rats that were 
exposed to 2, 2 dichlorovinyl dimethyl phosphate (DDVP) by inhalation. The animals were 
divided into seven groups, viz; unexposed control group and six groups exposed to DDVP 
for 1-6weeks in a poorly ventilated compartment. DDVP was prepared in a dilution of 1: 1 as 
recommended by manufacturer for domestic use. The exposed animals inhaled DDVP 
4hours daily for 1week, 2weeks, 3weeks, 4weeks, 5weeks and 6weeks. Animals were anaesthetized and blood drawn from the heart was used for haematological analysis using Sysmex kx21 auto-analyser. The result showed significant decreases in Mean Corpuscular Volume (MCV), Platelet Distribution Width (PDW), Mean Platelet Volume (MPV), Platelet Large Cell Ratio (P-LCR) of unexposed control rats compared with rats exposed for 5 weeks and 6 weeks. Significant increase in Mean Corpuscular Haemoglobin Concentration (MCHC) was observed in unexposed rats compared with rats exposed for 5 weeks and 6 weeks. There was also a significant decrease in haematocrit (HCT) of group exposed for 5 weeks when compared with unexposed group. There were no significant differences in MCV, PDW, MPV and P-LCR in unexposed rats compared with those exposed for 1week, 2weeks, 3weeks and 4 weeks. It was concluded from this study that long time inhalation of DDVP has negative effects on haematological indices; thus it must be used with caution.