HISTOLOGICAL AND BIOCHEMICAL CHANGES INDUCED BY ETHANOLIC LEAF EXTRACT OF MORINGA OLEIFERA IN THE HEART AND KIDNEYS OF ADULT WISTAR RATS (AKPOTOR)
Mr Akpotor Eugene Mudiaga and Ferdinand Uwaifo, Ejatuluchukwu Obi, Anthony Ngokere, Matthew Folaranmi Olaniyan, Bankole Henry Oladeinde (Published 2019)
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Abstract Study Background: Moringa oleifera, (miracle tree), has been used in the treatment of numerous diseases. Moringa has been
the subject of intensive scientific research; however, there have been scanty information
on its sub-acute effect on the kidneys.
Aims: This work was done to investigate the histological and biochemical effect of M. oleifera on the heart
and kidneys of adult Wistar rats.
Settings and Design: Twenty-eight adult healthy rats were used for this study. The rats were divided into
4 groups of 7 per group and fed with pellets and water ad libitum. Group A served as the control, Group B
were fed with 500 mg/kg of ethanolic extract of Moringa leaf, Group C with 1000 mg/kg while Group D
were fed with 1500 mg/kg body weight.
Methods and Material: Doses were administered once daily using oral gavage for 28 days. Before the
time of sacrificing the animals, blood samples were collected into plain test tubes and the animals
anesthetized before sacrifice. Necropsy was performed and the tissues (heart and kidney) processed and
stained using Hematoxylin and Eosin staining techniques. Serum electrolyte (sodium and potassium), urea,
creatinine, creatine kinase, and lactate dehydrogenase were analyzed for.
Results: There were significant increases in urea and potassium in the 1000 mg/kg (41.26 ± 16.89 and 10.52
± 1.48) and 1500 mg/kg (44.23 ± 17.49 and 13.82 ± 1.57) treated animals when compared with the control
(21.47 ± 16.33 and 4.54 ± 1.09). Histo-architecture of the kidneys showed glomerula atrophy in the 1000
mg/kg and 1500 mg/kg treated rats which is indicative of intrinsic renal damage. There were no noticeable
structural damage on the heart.
Conclusions: The result showed that M. oleifera may be toxic to the kidneys at concentrations higher than 1000 mg/kg.
|Item Type:||Journal article(non-copyrighted)|
|Format:||PDF document, 1.06 MB|
|Field of Study:||Nursing and Midwifery|
|Uploaded By:||Uwaifo Ferdinand|
|Date Added:||19 Feb 2019 12:49pm|
|Last Modified:||19 Feb 2019|
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