The success of applying bioherbicidal agents as biological control agents of weeds relies on the ability of the biological control agents to persist after its application and to remain viable after exposure to different environmental conditions. This research work was carried out to determine the persistence of bioherbicide formulated from multi-combination of the wild and mutant strain of Lasiodiplodia pseudotheobromae and Pseudomonas aeruginosa under field condition as well as soil carbon sequestration and soil functionality through the measurement of microbial biomass, total organic carbon and soil respiration. The viability of the multi formulated product was tested in two different trials after field at 3, 6 and 9 weeks after their application. The viability of the formulated bioherbicides was in the following orders:
BH4>BH2>BH6>BH3>BH1>BH5>Control. BH4 showed the maximum number of viability with 4.0 × 105 CFU/g and 4.2 × 105CFU/g at the two field trial, for Lasiodiplodia pseudotheobromae and 18.3 × 102 CFU/g and 23.48 × 102CFU/g for Pseudomonas aeruginosa, respectively after 12 weeks of application. The results revealed that multi-combination of Lasiodiplodia pseudotheobromae and Pseudomonas aeruginosa into different “Pasta” formulation greatly enhanced the viability of the bioherbicidal agent used during this study as well as carbon sequestration and microbial biomass at the two trial fields.