POSSIBLE ALTERATIONS IN THE PLASMA LEVELS OF PRO (TNF-ALPHA) AND ANTI-INFLAMMATORY (IL-10) CYTOKINES IN LONG-TERM INSOMNIA

Prof Olaniyan Mathew Folaranmi and Alade Ogunlade   (Published 2018)

Prof Mathew  Folaranmi
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Abstract

Abstract
Long-term/chronic insomnia occurs in patients for over one month; it can affect the functions of the immune system and responses, which may
involve cytokines. The aim was to determine alterations of Tumor necrosis factor alpha (TNF-alpha) and IL-10 cytokines in long-term insomnia.
Thirty-three long-term/chronic insomnia patients (male, 22; female, 11) aged 47-61 years were initially recruited. Seven of them who were
infected with microbial agents were excluded from the study. Of the 26 free of infectious agents only 21 (female, 5; male, 16) were successfully
monitored. Age-matched apparently healthy non-insomnia subjects (male, 25; female, 25) free of the infectious agents were recruited as
control. Plasma TNF-alpha, IL-10, HBsAg, anti-HCV, and HIVp24 antigen were assayed by ELISA method while determination of Mycobacterium
tuberculosis was by Ziehl–Neelsen staining of sputum and Plasmodium spp. by thick blood Giemsa staining. Of the 33 insomnia patients initially
recruited, 21.2% (7) were infected with microbial agents (Plasmodium spp., 6.1% (2); HCV, 3.0% (1); HBV, 6.1% (2); Mycobacterium tuberculosis,
3.0% (1); Plasmodium spp. + HBV, 3.0% (1); and HIVp24, 0). Twenty-one chronic insomnia patients were finally investigated on cytokines. There
were significantly higher mean plasma values of TNF-alpha and IL-10 cytokines in chronic insomnia patients before treatment than the values
obtained in the control subjects (p ? 0.05). The results also showed a significantly lower mean plasma value of TNF-alpha cytokine in chronic
insomnia patients after treatment than the values obtained in the patients before treatment. There were no significant differences in the mean
plasma values of IL-10 cytokines in chronic insomnia patients before and after treatment (p ? 0.05). There was a significant increase in plasma
TNF-alpha and IL-10 cytokines in long-term insomnia patients before treatment while plasma TNF-alpha significantly decreased after treatment,
and no significant difference was obtained in the plasma IL-10 before and after treatment. TNF-alpha could be a good investigative index of
insomnia.


Item Type: Journal article(non-copyrighted)
Format: PDF document,   637.17 KB
Copyright: Creative Commons LicenseCreative Commons license
Keywords: medical laboratory science
Department: Medical Laboratory Science
Field of Study: Medical Laboratory Science
Uploaded By: Uwaifo Ferdinand
Date Added: 08 Mar 2019 10:30am
Last Modified: 08 Mar 2019
Journal URL: https://www.edouniversity.edu.ng/oer/journal/possible_alterations_in_the_plasma_levels_of_pro_tnf-alpha_and_anti-inflammatory_il-10_cytokines_in_long-term_insomnia


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