Mr Ehiosun Kevin Iyere and Folasade M. Olajuyigbe, and Kikelomo F. Jaiyesimi   (Published 2015)

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Folasade M. Olajuyigbe, Kevin I. Ehiosun and Kikelomo F. Jaiyesimi (2015). Preliminary Study towards Enhanced Crude Oil Biodegradation Reveals Congeneric Total Peroxidases with Striking Distinctions. Advances in Enzyme Research, 3:66-74.



Peroxidases (POXs) are the key extracellular enzymes produced by crude oil degrading microbes. Knowledge of optimum conditions for POXs activity is crucial for providing effective environment for bioremediation. In this study, physicochemical properties of POXs produced by Actinomyces israelii and Actinomyces viscosus during growth on crude oil were studied. The POXs exhibited similarities in activity and stability with striking differences in response to two divalent metal ions. The POXs from both species had optimum pH of 7.0 and were very stable over a narrow pH range (6.0 - 8.0). The POXs demonstrated similar thermostability exhibiting relative residual activity of 62% at 50?C after 30 min incubation and 45% residual activity at the same temperature after 60 min despite the fact that POXs from A. viscosus and A. israelii had optimum temperatures of 50?C and 40?C, respectively. The POXs from A. viscosus and A. israelii were greatly activated by Fe2+ at 5.0 and 10.0 mM. The enzymes were both strongly inhibited by Cu2+, Mg2+ and Hg2+. Surprisingly, these congeneric POXs demonstrated striking differences in their response to Ca2+ and Mn2+. POX from A. viscosus was activated by Ca2+ and Mn2+ exhibiting relative activity of 136% and 106% at 5 mM, respectively. In contrast, POX from A. israelii was strongly inhibited by Ca2+ and Mn2+ exhibiting 62.5% relative activity in the presence of 5 mM of each metal ion. Increasing the concentration of Ca2+ and Mn2+ led to further activation of POX from A. viscosus and inhibition of POX from A. israelii. Results provide deeper insights into functional properties of studied POXs from closely
related microbes. The physicochemical properties are very similar; however, notable differences
provide a strong basis for structural characterization of these congeneric enzymes.

Keywords: Actinomyces israelii, Actinomyces viscosus, Bioremediation, Congeneric Enzymes, Crude Oil, Peroxidases

Item Type: Journal article(non-copyrighted)
Format: PDF document,   364.51 KB
Copyright: Creative Commons LicenseCreative Commons license
Keywords: Actinomyces israelii, Actinomyces viscosus, Bioremediation, Congeneric Enzymes, Crude Oil, Peroxidases
Department: Natural Science
Field of Study: Biochemistry
Uploaded By: Uddin Osemengbe Oyaimare
Date Added: 02 Nov 2017 12:45pm
Last Modified: 22 Nov 2017
Journal URL:

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