This work was performed in order to determine the effect of crude keratinase enzyme and the immobilized keratinase enzyme on the biodegradation of chicken feather wastes and to evaluate their effect on the growth of tomato plant. Moreover, the effect of variably formulated biofertilizer on soil enzymatic activity and soil carbon and soil respiration when compared to chemical fertilizer using different assay techniques. The order of bio-fertility activity is BCKE > BIKE >positive control. Additionally, the biofertilizer produced from the immobilized enzymes showed a significant difference on all the tested growth parameters like shoot length, root length, number of leaves, area of leaves and wet and dry biomass and enhanced the chlorophyll content of Solanum lycopersicum when compared to the control. After the 60th day period of experiment, the biofertilizer formulation from BCKE showed the highest value of 374 mg CO2 kg-1 soil hr-1 followed by BIKE with a value of 298 mg CO2 kg-1 soil hr-1 as against the positive control (NPK fertilizer) that had a value of 84 mg CO2 kg-1 soil hr-1 . The level of organic carbon content also showed that the biofertilizer treated soil exhibited increased soil carbon content and improved soil enzymatic activity when compared to the NKP treated soil. Therefore, this work has established that biofertilizer derived from microbial biodegradation of feather wastes could be utilized as a permanent replacement to the high cost and environmental unfriendly fertilizer. This will also go a long way in the total reduction of environmental pollution and a sustainable green technology for solid wastes management.