PROTECTIVE EFFECTS OF METHANOL EXTRACT OF PLUKENETIA CONOPHORA SEEDS AND 4H-PYRAN-4-ONE 2,3-DIHYDRO-3,5-DIHYDROXY-6-METHYL ON THE REPRODUCTIVE FUNCTION OF MALE WISTAR RATS TREATED WITH CADMIUM CHLORIDE
Dr Olaniyan Olugbemi Tope and Olugbemi Tope Olaniyan1,2, Olufadekemi Tolulope Kunle-Alabi2, Yinusa Raji2 (Published 2019)
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Objectives: Male infertility caused by exposure to
heavy metals is a current global issue. Exposure to cadmium
chloride (CdCl2) negatively affects the male reproductive
system. Many infertile people, especially in developing
countries, resort to folkloric treatment. Plukenetia
conophora is used in Nigerian folk medicine to promote
fertility. This study investigated the effects of Plukenetia
conophora (PC) and 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-
6-Methyl (DDMP) on Wistar rats with cadmium
chloride-induced testicular damage.
Methods: Forty-two male Wistar rats (150-190g) were
divided into seven groups (n=6) and treated daily for 54 days
as follows: Controls (normal saline); CdCl2 (2mg/kg single IP
dose); CdCl2 + 200 mg/kg vitamin E; CdCl2 + 100 or 200
mg/kg PC; and CdCl2 + 25 or 50 mg/kg DDMP. The rats were
sacrificed 55 days after the start of the study; Samples were
collected for analysis. Biochemical parame-ters
malondialdehyde, nitric oxide, antioxidant enzymes, and
proton pumps were measured by spectrophotometry.
Reproductive hormones were measured using ELISA. Data
were analysed using ANOVA and differences in mean val-ues
were considered significant at p<0.05.
Results: Significant increases in sperm count, motility,
and viability were observed in the groups given CdCl2+Vitamin
E, CdCl2+PC or CdCl2+DDMP as compared with the
CdCl2 group. Malondialdehyde and nitric oxide levels in the
groups treated with CdCl2+PC or CdCl2+DDMP decreased
significantly when compared with the group given CdCl2.
Significant increases were observed in antioxidant en-zymes,
proton pump, and testosterone in the groups treat-ed with
CdCl2+PC or CdCl2+DDMP, respectively.
Conclusion: Plukenetia conophora alleviated male reproductive
toxicity induced by cadmium chloride in Wistar
rats. 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-
Meth-yl present in Plukenetia conophora may be
responsible for the ameliorative effects.
|Item Type:||Journal article(non-copyrighted)|
|Format:||PDF document, 776.78 KB|
|Department:||Medicine and Surgery|
|Field of Study:||Health and Society|
|Uploaded By:||Uwaifo Ferdinand|
|Date Added:||06 Feb 2019 3:14pm|
|Last Modified:||06 Feb 2019|
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