Background: Transfusion-transmissible infectious agents such as hepatitis B virus (HBV),
human immunodeficiency virus (HIV), hepatitis C virus (HCV) and syphilis are among the
greatest threats to blood safety for transfusion recipients and pose a serious public health problem.
This cross-sectional study was undertaken with the aim of determining the seroprevalence of
HIV, HCV, hepatitis B surface antigen (HBsAg) and syphilis and correlates the findings with
sex and age to ascertain the associations, if any, in the occurrence of the pathogens.
Materials and Methods: HBsAg, antibodies to Treponema pallidum and HCV were
determined using Clinotech test strips. Antibodies to HIV types 1 and 2 were screened with
Determine and Immunocomb. All the reactive samples were confirmed using enzyme-linked
immunosorbent assays. Antibodies to Treponema pallidum were confirmed with a Treponema
pallidum haemagglutination test.
Results: A total of 1,410 apparently healthy prospective blood donors aged between 18 and
64 years (mean±SD, 32.58 ± 10.24 years) who presented for blood donation at the Ladoke
Akintola University of Technology Teaching Hospital Blood Bank, Osogbo were studied. The
male:female ratio was 6:1. Of the prospective blood donors, 406 (28.8%) had serological evidence
of infection with at least one infectious marker and 36 (2.6%) had dual infections. The overall
seroprevalence of HBsAg, HIV, HCV and syphilis was found to be 18.6%, 3.1%, 6.0% and
1.1%, respectively. The highest prevalences of HBsAg, HIV, HCV and syphilis infections occurred
among commercial blood donors and those aged 18 to 47 years old, the most sexually active age
group. There were no significant associations between pathogens except for syphilis and HIV
(p > 0.001).
Conclusion: The high seroprevalence of blood-borne pathogens among prospective blood
donors in Osogbo, Nigeria calls for mandatory routine screening of blood donors for HBV, HIV,
HCV and syphilis.